mouse anti syt2  (Developmental Studies Hybridoma Bank)

Journal: iScience

Article Title: Necl-1/CADM3 regulates cone synapse formation in the mouse retina

doi: 10.1016/j.isci.2024.109577

Figure Lengend Snippet: Necl-1 is expressed in OFF cone bipolar cells (A–E) Localization of the Necl-1 signal in the INL, OPL, and ONL. The retina was double-immunostained with the Necl-1 pAb (green) plus one of the following Abs for various retinal bipolar cell marker proteins (red): (A) an anti-HCN4 mAb for type 3a OFF CBCs; (B) an anti-PKARIIβ mAb for type 3b OFF CBCs; (C) an anti-calsenilin mAb for type 4 OFF CBCs; (D) an anti-SYT2 mAb for type 2 OFF CBCs and 6 ON CBCs; (E) an anti-PKCα mAb for rod BCs. For nuclear counter staining (blue), 4′,6-diamidino-2-phenylindole (DAPI) was used. IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; and ONL, outer nuclear layer. Scale bars in A–E, 15 μm. See also Figure S2 .

Article Snippet: Mouse monoclonal anti-SYT2 , Abcam , Cat# ab154035; RRID: AB_2916272.

Techniques: Marker, Staining

Journal: iScience

Article Title: Necl-1/CADM3 regulates cone synapse formation in the mouse retina

doi: 10.1016/j.isci.2024.109577

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-SYT2 , Abcam , Cat# ab154035; RRID: AB_2916272.

Techniques: Plasmid Preparation, Recombinant, Gel Extraction, PCR Cloning, Software

Journal: iScience

Article Title: Necl-1/CADM3 regulates cone synapse formation in the mouse retina

doi: 10.1016/j.isci.2024.109577

Figure Lengend Snippet: Necl-1 is expressed in OFF cone bipolar cells (A–E) Localization of the Necl-1 signal in the INL, OPL, and ONL. The retina was double-immunostained with the Necl-1 pAb (green) plus one of the following Abs for various retinal bipolar cell marker proteins (red): (A) an anti-HCN4 mAb for type 3a OFF CBCs; (B) an anti-PKARIIβ mAb for type 3b OFF CBCs; (C) an anti-calsenilin mAb for type 4 OFF CBCs; (D) an anti-SYT2 mAb for type 2 OFF CBCs and 6 ON CBCs; (E) an anti-PKCα mAb for rod BCs. For nuclear counter staining (blue), 4′,6-diamidino-2-phenylindole (DAPI) was used. IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; and ONL, outer nuclear layer. Scale bars in A–E, 15 μm. See also Figure S2 .

Article Snippet: The following antibodies used in this study were purchased from commercial sources: mouse anti-CtBP2 mAb (1:200) (BD Biosciences, San Jose, CA); goat anti-OPN1SW (S-opsin) pAb (1:500) (Santa Cruz Biotechnology, Dallas, TX); rabbit anti-red-green (L/M) opsin pAb (1:200) (Millipore, Burlington, MA); mouse anti-rhodopsin, RET-P1 (RHO) mAb (1:10,000) (Sigma-Aldrich Japan, Tokyo, Japan); mouse anti-HCN4 mAb (1:200) (Sigma-Aldrich Japan); mouse anti-PKARIIβ mAb (1:200) (BD Biosciences, San Jose, CA); mouse anti-SYT2 mAb (1:200) (Abcam, Cambridge, UK); mouse anti-calsenilin mAb (1:200) (Millipore); mouse anti-PKCα mAb (1:200) (Santa Cruz Biotechnology); mouse anti-calbindin mAb (1:3,000) (Sigma-Aldrich Japan); rabbit anti-calbindin pAb (1:2,000) (Millipore); mouse anti-GluR5 (GluK1) mAb (1:100) (Santa Cruz Biotechnology); rabbit anti-GluR1 (GluA1) pAb (1:200) (Abcam, Cambridge, UK); Alexa Fluor 594-conjugated lectin peanut agglutinin (PNA) (1:200) (Vector Laboratories, Burlingame, CA); anti-Chx10 pAb (1:200) (Santa Cruz Biotechnology); and rabbit anti-β-actin pAb (1:1,000) (Cosmo Bio, Tokyo, Japan).

Techniques: Marker, Staining

Journal: iScience

Article Title: Necl-1/CADM3 regulates cone synapse formation in the mouse retina

doi: 10.1016/j.isci.2024.109577

Figure Lengend Snippet:

Article Snippet: The following antibodies used in this study were purchased from commercial sources: mouse anti-CtBP2 mAb (1:200) (BD Biosciences, San Jose, CA); goat anti-OPN1SW (S-opsin) pAb (1:500) (Santa Cruz Biotechnology, Dallas, TX); rabbit anti-red-green (L/M) opsin pAb (1:200) (Millipore, Burlington, MA); mouse anti-rhodopsin, RET-P1 (RHO) mAb (1:10,000) (Sigma-Aldrich Japan, Tokyo, Japan); mouse anti-HCN4 mAb (1:200) (Sigma-Aldrich Japan); mouse anti-PKARIIβ mAb (1:200) (BD Biosciences, San Jose, CA); mouse anti-SYT2 mAb (1:200) (Abcam, Cambridge, UK); mouse anti-calsenilin mAb (1:200) (Millipore); mouse anti-PKCα mAb (1:200) (Santa Cruz Biotechnology); mouse anti-calbindin mAb (1:3,000) (Sigma-Aldrich Japan); rabbit anti-calbindin pAb (1:2,000) (Millipore); mouse anti-GluR5 (GluK1) mAb (1:100) (Santa Cruz Biotechnology); rabbit anti-GluR1 (GluA1) pAb (1:200) (Abcam, Cambridge, UK); Alexa Fluor 594-conjugated lectin peanut agglutinin (PNA) (1:200) (Vector Laboratories, Burlingame, CA); anti-Chx10 pAb (1:200) (Santa Cruz Biotechnology); and rabbit anti-β-actin pAb (1:1,000) (Cosmo Bio, Tokyo, Japan).

Techniques: Plasmid Preparation, Recombinant, Gel Extraction, PCR Cloning, Software